Scientific Leadership in IEF
CEPharm 2008

Application of Imaged CIEF in Clinical QC Testing
Protein Analytical Chemistry
Kimia Rahimi, John De Los Santos, Wenni Gao, Koman Joe and Sarah Du
Genentech, Inc., 1 DNA Way, South San Francisco CA 94080 USA

cIEF Method Development Highlights for a Monoclonal Antibody
Bioanalytical Development
Rob Killeen, Denise Lusitani, Shawn Russell and Jennifer Baker-Malcolm
Genzyme Corporation, Framingham, MA

Development of a Capillary Isoelectric Focusing Method for an Acidic Therapeutic Protein
Late Stage Analytical Development
James McElroy, David A. Michels and Oscar Salas-Solano
Genentech, Inc., 1 DNA Way, South San Francisco CA 94080 USA

icIEF as Analytical Tool for Recombinant Protein Charge Heterogeneity Analysis: From Development to Quality Control
Analytical Development
Zoran Sosic
Biogen Idec, Inc.

Imaged Capillary Isoelectric Focusing Analysis during Process Development of a Recombinant Fusion Protein
Liping Wang, Steve Davin, Geoff Lee, Gautam Nayer, Henrik Andersen
ZymoGenetics, Inc., 1201 Eastlake Avenue East, Seattle Washington USA

Imaged Capillary Isoelectric Focusing During Process Development and as a
Characterization Tool
Bioprocess Development
Liping Wang, Henrik Andersen
ZymoGenetics, Inc.

Influences of Carrier Ampholytes on the Separation of Proteins Observed with the
iCE280 IEF Analyzer
Tiemin Huang
Convergent Bioscience Ltd., Toronto ON M8Z 2L8 Canada

Method Development for the IgG Sample Used in the Inter-Company cIEF Collaboration (2008): Selection of Carrier Ampholytes
Jiaqi Wu
Convergent Bioscience Ltd., Toronto ON M8Z 2L8 Canada

Monitoring Charge Heterogeneity of Antibody-Maytansinoid Conjugates (AMC) with iCIEF
Joyce Lin, Rajesh Krishnamurthy, Alexandru Lazar
ImmunoGen, Inc., Waltham, MA

pI Measurement and Peak Identification in cIEF: Are Two pI Markers Enough? Jiaiqi Wu
Convergent Bioscience Ltd., Toronto ON M8Z 2L8 Canada

Successful Application of CE in Biologics Formulation Development Support
Worldwide Pharmaceutical Sciences, Biologics
Margaret Ruesch
Pfizer Inc., St. Louis MA

System and Calibration Marker Switchover in Imaged cIEF for Quality Control Testing of
Proteins by Isoelectric Profiling
Biopharmaceutical Development Program (BDP)
Gopalan Soman, Ph.D.
SAIC-Frederick, Inc.

Understanding Moving Peaks in cIEF
Tiemin Huang
Convergent Bioscience Ltd., Toronto ON M8Z 2L8 Canada